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SoftGenetics Products - Mutation Surveyor
Mutation Surveyor™ - A Unique Research
Tool
Mutation Surveyor is available in 400 and 48 lane capacities. Utilizing
our patented anti-correlation technology, the program rapidly locates
all differences between the wild type sequence and sample traces
with excellent accuracy and sensitivity. The program can be used
with either single direction or bi-directional data.
Analysis of Single and Bi-directional Data
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Single Direction
Analysis |
Dual Directional
Analysis |
Detection
Sensitivity to 5%
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Single as well
as dual directional sequence traces are easily analysed for
any potential variants using out patented anti-correlation
technology which performs an actual physical comparison of
the sample/patient traces to the reference traces. Found differences
are indicated by the sharp peaks in the mutation electropherogram.
Analysis courtesy of Dr. Yidong Bai, Assistant Professor,
University of Texas Health Centre, San Antonio TX. |
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Analysis courtesy
of Dr. Yidong Bai, Assistant Professor, University of Texas
Health Centre, San Antonio TX |
Accuracy of
the software in the bi-directional analysis mode is over 99%,
with sensitivity to greater than 5% of the primary peak. |
Researchers no longer need to perform time consuming and inaccurate
comparison of the entire sample trace. With Surveyor, any found
variants of the sample when compared to the wild type are clearly
indicated in our mutation electropherogram as a sharp peak.
Accuracy of the software in the bi-directional analysis mode is
over 99%, with sensitivity to greater than 5% of the primary peak.
Our collaborators have demonstrated an accuracy of 95% when processing
single direction sequence traces.
Surveyor’s detection sensitivity has been enhanced to report
similar peaks that are buried in baseline noise of both the forward
and reverse traces, alerting researchers to the possible presence
of mutations buried in the background noise.
Surveyor easily processes 400 lanes of data in approximately two
minutes, and can be operated on a fully automated unattended basis.
The software automatically forms contigs, performs alignments and
mutation detection comparing both forward and reverse patient traces
to reference or normal traces. Homozygote and Heterozygote mutations
are indicated by sharp peaks in our exclusive mutation electropherogram.
Unique algorithms perform a comparison of sample to reference traces
in both forward and reverse electropherograms checking for similar
peaks that are present in the background. This technique provides
almost analytical quality sensitivity.
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Homozygote Detection
Homozygote Variants are detected when the colour of the reference
drops 100% being replaced in the same spatial position by
a second colour in the sample or patient. |
Heterozygote Detection
The software detects heterozygote variants by observing a
drop in colour of in the sample vs. the surrounding peaks
and the reference, with a second wave length growing in approximately
the same proportion and in the same spatial position. |
Insertions and deletions are found by monitoring the mobility of
the sample DNA fragments, again in comparison to the reference,
to that of the reference, providing an extremely low false positive
rate and ignoring the miscalls or overcalls from basecaller software.
In the event that a heterozygote insertion or deletion is detected,
the software will de-convolute the multiple patient trace into two
clean traces, while continuing with the mutation detection.
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Dual Insertion |
Heterozygote insertion
/ deletion |
Surveyor and
Explorer monitor the migration time of the DNA fragments in
comparison to the references. If the program detects a change
in the migration time, the Centre line turns from green to
red indicating the presence of an insertion or deletion. The
software then re-aligns the traces, to calculate the Indel.
The position of the Indel is indicated by the heavy
red line. |
Assembly of a “whole gene” mutational analysis is easily
accomplished with Surveyor. To assemble the mutational analysis
of an entire gene, simply enter the Genbank data, pre-analysed mutation
projects, and the software will assemble and calculate an allele
frequency, providing the locations of the found variants in bp order.
Reporting is extremely user-friendly with several customer customisable
reporting formats, or customer can easily cut and paste areas of
interest to any graphics capable program such as Microsoft Word.
Reports can be easily exported in text, XML or HTML formats. Click
here to see examples of the reporting feature.
 Download
the DNA
Variant Analysis PDF 
(1.18Mb)
Download
the SoftGenetics
Application Notes PDF
(241Kb)
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