| ULTRA PURE Bio/dNTP’s™ are custom
manufactured to guarantee batch to batch reproducibility. This
commitment to quality is very important in assays where variables
have to be minimised. Each set is supplied with a ‘Quality Assurance’
certificate. The Bio/dNTP’s™ are provided in a
ready-to-use liquid form either as a convenient cocktail or with
each dNTP as a separate 25µM solution.
Specification
Solution |
Empirical
Formula |
Molecular
Weight |
dATP (2'
Deoxyadenosine-5'-triphosphate, Sodium) |
C10H14N5O12P3Na2.3H2O |
589.2 |
| dCTP (2'-Deoxycytidine-5'-triphosphate,
Sodium) |
C9H12N5O13P3Na2.H2O |
551.1 |
dGTP (2'-Deoxyguanosine-5'-triphosphate,
Sodium) |
C10H12N5O13P3Na3.H2O |
591.2 |
| dTTP (2'-Deoxythymidine-5'-triphosphate,
Sodium) |
C10H14N2O14P3Na3.2H2O |
583.09 |
dUTP (2'-Deoxyuridine-5'-triphosphate,
Lithium) |
C9H11N2O14P3Li4 |
491.9 |
|
Storage
| Short/long term: |
Store at -20°C |
| Short term: |
Diluted working solution may be stored at 4°C for
up to 4 weeks |
|
Purity
Bio/dNTP's™Set
A set of four vials containing 25µmoles of each deoxynucleotide
triphosphate, dATP, dCTP, dGTP, dTTP. The dNTP's are in solution
and are provided at a concentration of 100mM, pH7
DNA Polymerisation Mix
A DNA polymerisation mix containing equimolar amounts of dATP,
dCTP, dGTP and dTTP. The dNTP’s are in solution and are provided
at a concentration of 5mM. For primer extension procedure, 4µl
in a total of 100µl will give a final concentration of 200µM
of each dNTP.
dNTP Set
A set of four vials containing 2.5µmoles of each deoxynucleotide
triphosphate, dATP, dCTP, dGTP, dTTP. The dNTP's are in solution
and are provided at a concentration of 10mM, pH7
DNA Polymerisation Mix
A DNA polymerisation mix containing equimolar amounts of dATP,
dCTP, dGTP and dTTP. The dNTP’s are in solution and are provided
at a concentration of 20mM. For primer extension procedure, 1µl
in a total of 100µl will give a final concentration of 200µM
of each dNTP.
|
